Journal: Chemical Science
Article Title: Glycan–protein cross-linking mass spectrometry reveals sialic acid-mediated protein networks on cell surfaces †
doi: 10.1039/d1sc00814e
Figure Lengend Snippet: The protein interaction networks revealed from the PNT2 cell line. (a) The network was constructed based on the glycoproteins (red) interacting with other proteins through their glycans. The size of the circle is proportional to the number of proteins involved in the interaction. (b) A network corresponding to a protein that interacted with glycoproteins through other protein glycans. The glycan linkage was annotated based on Hex ( n ) HexNAc ( n ) Fuc ( n ) Sia ( n ) and colored as in the legend.
Article Snippet: Human immortalized normal prostatic epithelial PNT2 cells were obtained from ATCC and grown in Roswell Park Memorial Institute (RPMI) 1640 medium, while supplemented with 10% (v/v) fetal bovine serum (FBS), 100 U ml −1 penicillin and 100 μg ml −1 streptomycin.
Techniques: Construct, Glycoproteomics